The ocean is the largest independent ecosystem in the world, including deep seawater, surface sediments, cold springs, hydrothermal, polymetallic nodule areas and other geological structures, 70% of the seawater is in a high pressure environment above 38 MPa. The microorganisms in deep-sea biosphere account for 90% of the total microorganisms in the world, and pressure resistance is its distinctive feature, at the same time, the hydrothermal vent brings high temperature and the flow of reducing substances such as hydrogen rich and sulfide. Therefore, in order to carry out in-situ research on the deep-sea deep biosphere in the laboratory, we need to solve a series of technical problems such as fidelity sampling, fidelity transfer, high temperature/low temperature fidelity culture under high pressure, in situ analysis and so on. Our company's series of products can provide powerful help for this research.

1. Fidelity sampling

A. Fidelity sampling bottle:

Titanium alloy

500ml effective sampling volume

120MPa fidelity sampling pressure

0~200ºC sampling temperature range

B. Pressure self-balancing system:

The volume of buffer water collection tank is 500 ml.

Pressure variation in sampling bottle during balancing rise of pressure compensator

With the bottle pressure sensor, working pressure 0-120MPa, pressure resolution 0.1MPa, battery life 10 hours.

2. Sampling mechanism

Used for fixing and connecting the above components to the lander, including sampling valve release hook, sampling valve, one-way stop valve, etc.

The user can mount the fidelity sampling system through a multi-channel water collection system which shares the trigger mechanism with the multi-channel water collection system.It is suitable for fidelity sampling at different depths in a dive mission. Also time series sampling, for example, the fidelity sampling system lander is mounted on the bottom to make it work continuously for one year, and a set of fidelity sampling bottles is opened every month for sample collection. After one year, the mother ship recovered 12 sets of fidelity sampling systems for comparison.

3. Fidelity transfer

A. High pressure metering pump

Single cylinder volume: 250ml

Control pressure: 150MPa

Pressure control accuracy: ±0.1MPa

Injection measurement accuracy: ±0.01ml

Driving mode: Double cylinder continuous injection

B. High pressure transfer platform

Transfer platform working pressure: 150MPa

Pressure sensor accuracy: ±0.1MPa

Fidelity transfer bottle volume: 50ml

4. Fidelity culture

A. High pressure gas distribution system

Multi-gas unit: CO2, CH4, H2, N2

Booster pump pressure: 150MPa

Gas storage buffer tank volume: 1000ml

Deoxygenation circuit: Yes

Pressure sensor accuracy: ±0.1MPa culture solution preparation system

B. Culture solution preparation system

High pressure mixed phase reactor/Solid quantitative feed/Liquid quantitative feed/Sterilization device

Four channel air intake device

Volume: 2L

Working pressure: 50MPa

Working temperature: 250°C

Magnetic coupling stirring torque: 200Ncm

C. Culture solution quality monitoring / analysis

The dissolution of the high pressure gas in the culture solution is monitored in real time.

Composition analysis and quality evaluation of culture solution under specific temperature and pressure conditions.

D. Culture solution delivery system

High precision constant current / constant pressure injection pump

Pump cylinder material: titanium alloy

High pressure culture autoclave volume: 250ml

Control pressure: 150MPa

Pressure control accuracy: ±0.05% F.S.

Injection measurement accuracy: ±0.01ml

E. High pressure control pressure pump set

Control pressure: 150MPa

Pressure control accuracy: ±0.5MPa

Flow rate: 1000ml/min

Boost rate: 20MPa/min

Depressurization rate: 2MPa/min

F. Hydrothermal culture autoclave set

Autoclave material: Titanium alloy

Volume specification: 1l~ 20l

Working pressure: 150MPa

Working temperature: Room temperature ~ 300°C

Temperature control accuracy: ± 0.5°C

Program controlled sampling

G. Cold springs culture autoclave set

Autoclave material: Titanium alloy

Volume specification: 1l~ 20l

Working pressure: 150MPa

Working temperature: 0°C~room temperature

Temperature control accuracy: ± 0.1°C